litaba

Li-alkaloid tsa Toluidine tse kang anisodamine le cyperine li na le mesebetsi ea bohlokoa ea bioloji le boleng ba meriana ea bongaka. Li-alkaloids tsena li fumanoa haholo-holo ke mekhoa ea moetlo ea ho ntša limela tsa mantlha, 'me theknoloji ea mahlale a heterologous e thehiloeng ho biology ea maiketsetso e fana ka leano le lecha la ho hlophisa tlhaho ea moriana Ho biosynthesis ea n-methylpyrrolidine, l-ornithine e ne e sebelisoa e le selelekela, se ileng sa ts'oaroa ke ornithine.

safds

Bakeng sa ho aha lisele tsa heterologous le synthesis ea sehlopha sa N - methyl pyrroline chassis XiaoYouLi sehlopha se bataletseng le sehlopha sa lipatlisiso sa xiao-dong li zhou, bafuputsi ba entse lipatlisiso tse latelang: 1) mohloli oa pele oa semela sa moriana lintlha tse tharo AaDAO2 amine oxidase le Ts'ebetso ea biochemical ea AaDAO3, mohloli le ho kopanya selekane sa coca ornithine decarboxylase EcODC le putrescine mehloli ea anisodus tanguticus - N - methyl transferase ho pitsa ea vitro enzyme catalysis synthesis le ho lekola EcODC, AtPMT le AaDAO3 in vitro synthesis of N - methyl pyrticine optimal ; 2) liphatsa tsa lefutso tse tharo mojuleng ona li ile tsa hlahisoa hape ho escherichia coli le saccharomyces cerevisiae ho aha lisele tsa chassis tse tsamaellanang le ho etsa tlhahlobo ea ho belisa e tsitsipanyang. Sepheo sa n-methylpyrrolium se fumanoe ka katleho ka lihlahisoa tsa 3.02 le 2.07mg / L ka ho latellana. 3) motheong oa sena, molemong oa ho eketsa tlhahiso, lipatlisiso li boetse lia kokota ntle le chassis ea chassis ho N - tlholisano ea methyl pyrroline ea methapo e amanang le li-enzyme ALD4, ALD5 le HFD1, ka nako e ts'oanang SAM2 e matlafalitsoe ka enzyme ea senotlolo ea AtPMT e thusang ho kopanya li-cofactors tse hlokahalang bakeng sa SAM, qetellong tomoso lisele tsa chassis ho N - methyl pyrroline e fihletse 17.82 mg / L ha e bapisoa le khatello ea mantlha e eketsehile makhetlo a 8.6.

Phuputso ena e bile ea pele ea ho aha sele ea chassis ea microbial bakeng sa heterologous synthesis ea n-methylpyrrolidine, e ileng ea rala motheo oa bohlokoa oa tlhahlobo le heterologous synthesis ea meriana alkaloids pele ho n-methylpyrrolidine.

Phuputso e boetse e tšehelitsoe ke univesithi ea Zhejiang ea moprofesa oa setso oa bongaka oa Chaena zhang chuanyin mme e tšehelitsoe ke Sekolo sa Mahlale sa China. ea setsi sa bokhabane ba semela sa limolek'hule


Nako ea poso: Mots'eanong-28-2020